分子量:52 kDa

纯度:>95% as determined by SDS-PAGE.

内毒素:Less than 1.0 EU per μg of the Mouse Carboxypeptidase E, His Tag by the LAL method.

Buffer:PBS, pH7.4

生物活性:Measured by its ability to cleave a peptide substrate, benzoylAROH. The product, Arg, is reacted with orthophthaldialdehyde (OPA) to form a fluorescent molecule.  The specific activity is > 12000 pmoles/min/μg

产品特性:Mouse Carboxypeptidase E, His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 52 kDa. The predicted N-terminus is Gln 28. The reducing (R) protein migrates as 52 kDa in SDS-PAGE .

产品背景:Carboxypeptidase E (CPE) is also known as Carboxypeptidase H or enkephalin convertase. Carboxypeptidase E cleaves C-terminal amino acid residues and is involved in neuropeptide processing. It is a peripheral membrane protein. CPE specifically binds regulated secretory pathway proteins, including prohormones, but not constitutively secreted proteins. Carboxypeptidase E appears to have several functions. The active form of carboxypeptidase E was shown to be in secretory vesicles, where it acts as an exopeptidase to activate neuropeptides, It does that by cleaving off basic C-terminal amino acids, producing the active form of the peptide. Products of carboxypeptidase E include insulin, enkephalin, and most other neuroendocrine peptides.It has also been proposed that membrane-associated carboxypeptidase E acts as a sorting signal for regulated secretory proteins in the trans-Golgi network of the pituitary and in secretory granules; regulated secretory proteins are mostly hormones and neuropeptides. Mice with mutant carboxypeptidase E Cpe, display endocrine disorders like obesity and infertility.