Overview

Product name
Cathepsin K Activity Assay Kit (Fluorometric)


Product overview
Apoptosis can be mediated by mechanisms other than the traditional caspase-mediated cleavage cascade. There is growing recognition that alternative proteolytic enzymes such as the lysosomal cathepsin proteases may initiate or propagate proapoptotic signals.

Cathepsins are lysosomal enzymes that are also used as sensitive markers in various toxicological investigations. 

The Cathepsin-K Activity Assay kit is a fluorescence-based assay that utilizes the preferred cathepsin-K substrate sequence LR labeled with AFC

(amino-4-trifluoromethyl coumarin). Cell lysates or other samples that contain cathepsin K will cleave the synthetic substrate LR-AFC to release free AFC. The released AFC can easily be quantified using a fluorometer or fluorescence plate reader. The cathepsin K assay is simple, straightforward, and can be adapted to 96-well plate assays. Assay

conditions have been optimized to obtain the maximal activity.


Notes
Store kit at –20oC (Store Cell Lysis Buffer and Reaction Buffer at 4oC after opening). Protect the Substrate from light. All reagents are stable for 6 months under proper storage conditions.


Tested applications
Functional Studiesmore details

Properties

Storage instructions
Store at -20°C, Stable for 6 months at -20°C



    

        

            
Components
            
Identifier
            
100 tests
        
    
    

        

            
Cathepsin K Activity Assay Kit (Fluorometric)
            
AB65303
            
1 x 100 kits
        
        

            
CK Cell Lysis Buffer
            
WM
            
1 x 25ml
        
        

            
CK Inhibitor (1mM)
            
Red
            
1 x 1µl
        
        

            
CK Reaction Buffer
            
NM
            
1 x 5ml
        
        

            
CK Substrate Ac-LR-AFC (10mM)
            
Amber
            
1 x 200µl
        
    


http://www.abcam.com/cathepsin-k-activity-assay-kit-fluorometric-ab65303.html