分子量:42.2 kDa

纯度:>95% as determined by SDS-PAGE.

内毒素:Less than 1.0 EU per μg of the Biotinylated Human CD47, Fc Tag by the LAL method.

Buffer:50 mM tris, 100 mM glycine, pH7.5

生物活性:Measured by its binding ability in a functional ELISA.Immobilized Human SIRP alpha Protein, His Tag (Cat# SIA-H5225 ) at 2 μg/mL (100 μL/well) can bind Biotinylated Human CD47 Protein, Fc Tag (Cat# CD7-H82F6 ) with a linear range of  8-250 ng/mL.

产品特性:This protein carries a a 15 AA fragment ,containing one single lysine residue that is enzymatically labeled with biotin in vivo, at the C-terminus, followed by a human IgG1 Fc fragment. The protein has a calculated MW of 42.2 kDa. The predicted N-terminus is Gln 19. The reducing (R) protein migrates as 50 kDa in SDS-PAGE due to glycosylation.

产品背景:Leukocyte surface antigen CD47 is also known as Antigenic surface determinant protein OA3, Integrin-associated protein (IAP) and Protein MER6. CD47 contains 1 Ig-like V-type (immunoglobulin-like) domain. CD47 is very broadly distributed on normal adult tissues. CD47 has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins and plays an important role in memory formation and synaptic plasticity in the hippocampus by similarity. CD47 is the receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. CD47 Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation.