GUF1抗体-抗体-抗体-生物在线
GUF1抗体

GUF1抗体

商家询价

产品名称： GUF1抗体

英文名称： GUF1Antibody

产品编号： XY17489-1

产品价格： null

产品产地： 中国/美国

品牌商标： XYbscience

更新时间： 2023-08-17T09:55:27

使用范围： WB,ELISA,IHC-P,IHC-F,IF

上海信裕生物科技有限公司
  • 联系人 : 徐经理
  • 地址 : 上海市闵行莘庄工业区春东路508号A1-2F
  • 邮编 : 200612
  • 所在区域 : 上海
  • 电话 : 152****8802
  • 传真 : 021-37680378
  • 邮箱 : shxysw02@163.com
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 GUF1抗体

Applications
Tested Applications: ELISA, WB, IF, IHC, IP
Specificity Reaction: Human, Mouse; Other species are not tested.Please decide the specificity by homology
Positive WB detected in HeLa cells, HepG2 cells, mouse brain tissue, mouse liver tissue, mouse pancreas tissue
Positive IP detected in HeLa cells
Positive IHC detected in Human oesophagus tissue, human kidney tissue, human lung cancer tissue, human testis tissue
Positive IF detected in HepG2 cells
Recommended dilution WB : 1:500-1:5000
IP : 1:200-1:2000
IHC : 1:20-1:200
IF : 1:10-1:100
 GUF1抗体Application key: WB=Western Blotting ,IHC=Immunohistochemistry, IF=Immunofluorescence
Images
Prev12NextView more validation data
  • 17489-1-AP;HeLa cellsHeLa cells were subjected to SDS PAGE followed by western blot with 17489-1-AP(GUF1 antibody) at dilution of 1:500
  • 17489-1-AP;human oesophagusImmunohistochemical of paraffin-embedded human oesophagus using 17489-1-AP(GUF1 antibody) at dilution of 1:50 (under 10x lens)
  • 17489-1-AP;human oesophagusImmunohistochemical of paraffin-embedded human oesophagus using 17489-1-AP(GUF1 antibody) at dilution of 1:50 (under 40x lens)
  • 17489-1-AP;HepG2Immunofluorescent analysis of HepG2 cells, using GUF1 antibody 17489-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
  • 17489-1-AP;HeLa cellsIP Result of anti-GUF1 (IP:17489-1-AP, 3ug; Detection:17489-1-AP 1:500) with HeLa cells lysate 3200ug.
Product Information GUF1抗体
Source: Rabbit Purify: Antigen affinity purification
IsoType: IgG Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3. -20oC, Avoid freeze / thaw cycles.
Immunogen Information
Immunogen: GUF1 fusion protein ag11579 Full Name: GUF1 GTPase homolog (S. cerevisiae)
Caculated Molecular
Weight:		 669aa,74 kDa Observed Molecular 
Weight:		 70 kDa
GenbankNo BC036768 GeneID: 60558
Symbol GUF1 Synonyms FLJ13220, GUF1
Background

GUF1 (GUF1 GTPase), also known as EF-4 (elongation factor 4 homolog), is a 669 amino acid protein belonging to the GTP-binding elongation factor family and the LepA subfamily. Localizing to the mitochondrion inner membrane, GUF1 promotes mitochondrial protein synthesis and binds to mitochondrial ribosomes in a GTP-dependent manner. GUF1 may act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. In western blotting, we got double bands 74 kDa and 70 kDa, due to the N-terminal 1-49 amino acid sequence that could be cut down. GUF1抗体

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