分子量:6.3 kDa

纯度:>97% as determined by SDS-PAGE.

内毒素:Less than 1.0 EU per μg of the Human EGF, Tag Free by the LAL method.

Buffer:PBS, pH7.4

生物活性:Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblasts.The ED50 for this effect is typically 20-100 pg/mL.
Measured by its binding ability in a functional ELISA. 
Immobilized Human EGF, Tag Free (Cat# EGF-H4114)  at 10μg/mL (100 l/well),can bind Human EGF R Protein (Cat# EGR-H5222) with a linear of 0.2-2.5 μg/mL.


产品特性:Human EGF, Tag Free contains no "tag", and has a calculated MW of 6.3 kDa. The reducing (R) protein migrates as 6.3 kDa in SDS-PAGE .

产品背景:Human epidermal growth factor (EGF) is also known as HOMG4 and URG,and is a growth factor that plays an important role in the regulation of cell growth, proliferation, and differentiation by binding to its receptor EGFR. Epidermal growth factor can be found in human platelets, macrophages, urine, saliva, milk, and plasma. EGF is the founding member of the EGF-family of proteins. Members of this protein family have highly similar structural and functional characteristics. All family members contain one or more repeats of the conserved amino acid sequence. The biological effects of salivary EGF include healing of oral and gastroesophageal ulcers, inhibition of gastric acid secretion, stimulation of DNA synthesis as well as mucosal protection from intraluminal injurious factors such as gastric acid, bile acids, pepsin, and trypsin and to physical, chemical and bacterial agents. Because of the increased risk of cancer by EGF, inhibiting it decreases cancer risk.