分子量:71.9 kDa

纯度:>97% as determined by SDS-PAGE.

内毒素:Less than 1.0 EU per μg of the C13 and N15-labeled, Human PCSK9 by the LAL method.

Buffer:PBS, pH7.4

生物活性:

产品特性:C13 and N15-labeled, Human PCSK9 is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 71.9 kDa. The predicted N-terminus is Gln 31. The reducing (R) protein migrates as 20 kDa and 62 kDa in SDS-PAGE  due to glycosylation and proteolytic digestion.

产品背景:Proprotein convertase subtilisin/kexin type 9 (PCSK9) is also known as NARC1 (neural apoptosis regulated convertase), is a newly identified subtilase belonging to the peptidase S8 subfamily. Mouse PCSK9 is synthesized as a soluble zymogen, and undergoes autocatalytic intramolecular processing in the endoplasmic reticulum, resulting in the cleavage of its propeptide that remains associated with the secreted active enzyme with a broad alkaline pH optimum. This protein plays a major regulatory role in cholesterol homeostasis. PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of the low-density lipoprotein receptor (LDLR), inducing LDLR degradation. PCSK9 may also have a role in the differentiation of cortical neurons. Mutations in this gene have been associated with a rare form of autosomal dominant familial hypercholesterolemia (HCHOLA3).