Overview

Product name
GST Activity Colorimetric Assay Kit

See all GST products (2) ...


Product overview
The GST Colorimetric Activity Assay Kit is based upon the GST-catalyzed reaction between GSH and the GST substrate, CDNB (1-chloro-2,4-dinitrobenzene, which has the broadest range of isozyme detectability (e.g., alpha-, mu-, pi-, and other GST isoforms). Under certain conditions, the interaction between glutathione and CDNB is totally dependent on the presence of active GST.

The GST-catalyzed formation of CDNB-GSH produces a dinitrophenyl thioether which can be detected by spectrophotometer at 340 nm. One unit of GST activity is defined as the amount of enzyme producing 1 mmol of CDNB-GSH conjugate/min under the conditions of the assay. The kit can detect GST activity in crude cell lysate or purified protein fraction, and also quantitate GST-tagged fusion protein. Detect limit: Active GST 10 ng/assay.


Notes
GST Assay Buffer: May be stored at 4°C.

GSH: Add 275 µl of GST Assay Buffer to each vial just before use. One vial is sufficient for 50 assays. The Remaining solution can be kept at –20°C for 1 week.

CDNB: This vial contains a DMSO solution of 1-chloro-2, 4-dinitrobenzene (CDNB) and should be stored at -20°C.

GST Positive Control: Store at -20°C


Tested applications
Functional Studiesmore details

Properties

Storage instructions
Please see Notes section



    

        

            
Components
            
Identifier
            
100 tests
        
    
    

        

            
Glutathione (GSH, lyophilized)
            
Green
            
2 x 17mg
        
        

            
GST Activity Colorimetric Assay Kit
            
ab65326
            
1 kit
        
        

            
GST Assay Buffer
            
NM
            
1 x 25ml
        
        

            
GST Positive Control (0.625 µg/µl)
            
Yellow
            
1 x 20µl
        
        

            
GST Substrate (CDNB)
            
Red
            
1 x 0.5ml
        
    


http://www.abcam.com/GST-Activity-Colorimetric-Assay-Kit-ab65326.html