分子量:36.6 kDa

纯度:>98% as determined by SDS-PAGE.

内毒素:Less than 1.0 EU per μg of the Human CD14, His Tag by the LAL method.

Buffer:PBS, pH7.4

生物活性:Measured by its ability to enhance LPS stimulated IL8 secretion by THP-1 human acute monocytic leukemia cells. The ED50 for this effect is typically 60-120 ng/mL.

产品特性:Human CD14, His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 36.6 kDa. The predicted N-terminus is Thr 20. The reducing (R) protein migrates as 45-50 kDa in SDS-PAGE  due to glycosylation.

产品背景:Cluster of differentiation 14 (CD14), is a cell surface glycoprotein, and is a is a component of the innate immune system. CD14 is a myelomonocytic differentiation antigen preferentially expressed on monocytes, macrophages, and activated granulocytes. CD14 exists in two forms. Either it is anchored into the membrane by a glycosylphosphatidylinositol tail (mCD14) or it appears in a soluble form (sCD14). Soluble CD14 either appears after shedding of mCD14 (48 kDa) or is directly secreted from intracellular vesicles (56 kDa). CD14 acts as a co-receptor (along with the Toll-like receptor TLR 4 and MD-2) for the detection of bacterial lipopolysaccharide (LPS). CD14 can bind LPS only in the presence of lipopolysaccharide-binding protein (LBP). CD14 has been proposed to be involved in various biological processes, including transportation of other lipids, cell-cell interaction during different immune responses, as well as recognition of apoptotic cells. Although LPS is considered its main ligand, CD14 also recognizes other pathogen-associated molecular patterns. CD14+ cells are monocytes that can differentiate into a host of different cells. CD14 has been shown to interact with Lipopolysaccharide-binding protein.