官方网址：http://www.sinobiological.cn/Von-Willebrand-Factor-vWF-Antibody-g-520.html
Von Willebrand Factor ( vWF ) Antibody
 
 
 
 
 
 
 
 
 
Order or Inquire for vWF Antibody product
 
 
 
 
 
 
 
Detection limit is 20 ng/lane in WB
 
 
 
 
 
 
vWF抗体产品信息
 
 
 
 
免疫原 : 
 
 
Recombinant human vWF protein (  Catalog#10973-H08C )
 
 
 
 
抗体类型: 
 
Mouse Monoclonal Antibody ( Mouse mAb Service Platform )
 
 
 
 
Clone ID :
 
 
 
5G4D3H2
 
 
 
 
抗体宿主 :
 
 
Mouse IgG2a
 
 
 
 
缓冲液 :
 
0.2 μm filtered solution in PBS with 5% trehalose
 
 
 
制备方法 : 
 
 
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, CHO cell-derived, recombinant human von Willebrand factor (rhvWF; Catalog#10973-H08C; aa 1- 2813; NP_000543.2). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
 
 
 
 
 
vWF抗体使用指南
 
 
 
 
特异性 : 
 
 
Human vWF
 
 
 
 
 
 
 
No cross-reactivity with human cell lysate ( 293 cell line ) in WB and ELISA
 
 
 
 
Western blot : 
 
This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect human vWF in WB. Using a DAB detection system, the detection limit for human vWF is approximately 8ng/lane under non-reducing conditions and 20 ng/lane under reducing conditions.
 
 
 
储存方法 :
 
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
 Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly.  Avoid repeated freeze-thaw cycles.
 
 
 
 
vWF Antibody Related Products & Topics
 
 
相关研究领域:
 
Stem Cell>>Hematopoietic Stem Cell (HSC)>>Hemangioblast>>Endothelial Cell Marker>>VWF
 
Immunology>>Innate Immunity>>Coagulation>>VWF
 
蛋白:
 
 
 
 
Molecule
 
Species
 
Description //For Detailed Info.------CLICK!
 
Cat No
 
 
 
VWF
 
Human
 
VWF Protein, Recombinant  
 
10973-H08C
 
 
 
 
抗体:
 
 
 
 
Molecule
 
Application
 
Description //For Detailed Info.------CLICK!
 
Cat No
 
 
 
Human VWF
 
WB 
 
Mouse Monoclonal Antibody
 
10973-MM01
 
 
 
Human VWF
 
ELISA  
 
Mouse Monoclonal Antibody
 
10973-MM02
 
 
 
Human VWF
 
WB, ELISA
 
Rabbit Monoclonal Antibody
 
10973-R111
 
 
 
Human VWF
 
WB, ELISA
 
Rabbit Polyclonal Antibody
 
10973-RP03
 
 
 
Human VWF
 
WB, ELISA
 
Rabbit Polyclonal Antibody (Antigen Affinity Purified)
 
10973-RP04
 
 
 
 
 
 vWF Antibody Background
 
 
Von Willebrand factor (vWF) is a blood glycoprotein which is involved in a large number of diseases, including thrombotic thrombocytopenic purpura, Heyde's syndrome, and possibly hemolytic-uremic syndrome. Its primary function is binding to other proteins, particularly Factor VIII and it is important in platelet adhesion to wound sites. vWF appears to play a major role in blood coagulation. Its deficiency or dysfunction leads to a bleeding tendency, which is most apparent in tissues having high blood flow shear in narrow vessels. It appears that vWF uncoils under these circumstances, decelerating passing platelets. Hereditary or acquired defects of vWF lead to von Willebrand disease (vWD), a bleeding diathesis of the skin and mucous membranes, causing menorrhagia, and gastrointestinal bleeding.  
 
 
参考文献
 
 
 
Sadler, J.E. et al., 1998. Annu. Rev. Biochem. 67: 395-424.
 
Levy, G. G. et al., 2005, Blood. 106 (1): 11-17.
 
Sadler, J. E. et al., 2006, J. Thromb. Haemost. 4 (10): 2103-2114.
 
 
 
 
官网链接：http://www.sinobiological.com/Von-Willebrand-Factor-vWF-Antibody-g-520.html